Publikationen
2021
Nerlich, C., Epalle, N.H., Seick, P., Beitz E. (2021) Discovery and development of inhibitors of the plasmodial FNT-type lactate transporter as novel antimalarials. Pharmaceuticals 14: 1191. PubMed
Jakobowska, I., Becker, F., Minguzzi, S., Hansen, K., Henke, B., Epalle, N.H., Beitz, E., Hannus, S. (2021) Fluorescence cross-correlation spectroscopy yields true affinity and binding kinetics of Plasmodium lactate transport inhibitors. Pharmaceuticals 14: 757 PubMed
Köpnick, A.L., Geistlinger, K., Beitz, E. (2021) Cysteine 159 delineates a hinge region of the alternating access monocarboxylate transporter 1 and is targeted by cysteine-modifying inhibitors. FEBS J. 288:6052-6062. PubMed
Beitz, E. (2021) Structure memes: Intuitive visualization of sequence logo and subfamily logo information in a 3D protein-structural context. Proteins 89: 1262-1269. PubMed
Pfeifer, L., Baumann, A., Petersen, M.L., Höger, B., Beitz, E., Classen, B. (2021) Degraded arabinogalactans and their binding properties to cancer-associated human galectins. Int. J. Mol. Sci. 22: 4058. PubMed
Köpnick, A.L., Jansen, A., Geistlinger, K., Epalle, N.H., Beitz, E. (2021) Basigin drives intracellular accumulation of L-lactate by harvesting protons and substrate anions. PLoS One 16: e0249110. PubMed
Schmidt, J.D.R., Walloch, P., Höger, B., Beitz, E. (2021) Aquaporins with lactate/lactic acid permeability at physiological pH conditions. Biochimie 188: 7-11. PubMed
Beitz, E. (2021) Aquaporins. In: Offermanns S., Rosenthal W. (eds) Encyclopedia of Molecular Pharmacology. Springer, Cham. Springer Link
Walloch, P., Hansen, C., Priegann, T., Schade, D., Beitz, E. (2021) Pentafluoro-3-hydroxy-pent-2-en-1-ones potently inhibit FNT-type lactate transporters from all five human-pathogenic Plasmodium species. ChemMedChem 16: 1283-1289. PubMed
2020
Petersen, L.M., Beitz, E. (2020) The ionophores CCCP and gramicidin but not nigericin inhibit Trypanosoma brucei aquaglyceroporins at neutral pH. Cells 9: 2335. PubMed
Bader, A., Beitz, E. (2020) Transmembrane facilitation of lactate/H+ instead of lactic acid is not a question of semantics but of cell viability. Membranes 10: E236. PubMed
Walloch, P., Henke, B., Häuer, S., Bergmann, B., Spielmann, T., Beitz, E. (2020) Introduction of scaffold nitrogen atoms renders inhibitors of the malarial L-lactate transporter, PfFNT, effective against the Gly107Ser resistance mutation. J. Med. Chem. 63: 9731-9741. PubMed
Kreiter, J., Beitz, E., Pohl, E.E. (2020) A fluorescence-based method to measure ADP/ATP exchange of recombinant adenine nucleotide translocase in liposomes. Biomolecules 10: E685. PubMed
2019
Hajek, P., Bader, A., Helmstetter, F., Henke, B., Arnold, P., Beitz, E. (2019) Cell-free and yeast-based production of the malarial lactate transporter, PfFNT, delivers comparable yield and protein quality. Front. Pharmacol. 10: 375. PubMed
Helmstetter, F., Arnold, P., Höger, B., Petersen, L.M., Beitz, E. (2019) Formate-nitrite transporters carrying nonprotonatable amide amino acids instead of a central histidine maintain pH-dependent transport. J. Biol. Chem. 294: 623-631. PubMed
2018
Erler, H., Ren, B., Gupta, N., Beitz, E. (2018) The intracellular parasite Toxoplasma gondii harbors three druggable FNT-type formate and L-lactate transporters in the plasma membrane. J. Biol. Chem. 293: 17622-17630. PubMed
Uzcategui, N.L., Figarella, K., Segninia, A., Marsiccobetrea, S., Lang, F., Beitz, E., Rodriguez-Acosta, A., Bertl, A. (2018) Trypanosoma brucei aquaglyceroporins mediate the transport of metabolic end-products: methylglyoxal, D-lactate, L-lactate and acetate. Biochim. Biophys. Acta Biomembr. 1860: 2252-2261. PubMed
Meier, A., Erler, H., Beitz, E. (2018) Targeting channels and transporters in protozoan parasite infections. Front. Chem. 6: 88. PubMed
2017
Wiechert, M., Erler, H., Golldack, A., Beitz, E. (2017) A widened substrate selectivity filter of eukaryotic formate-nitrite transporters enables high-level lactate conductance. FEBS J. 284: 2663-2673. PubMed
Wiechert, M., Beitz, E. (2017) Formate-nitrite transporters: monoacids ride the dielectric slide. Channels 11: 365-367. PubMed
Rothert, M., Rönfeldt, D., Beitz, E. (2017) Electrostatic attraction of weak monoacid anions increases probability for protonation and passage through aquaporins. J. Biol. Chem. 292: 9358-9364. PubMed
Scheltz, T., von Bülow, J., Beitz, E. (2017) Reducing isoform complexity of human tetraspanins by optimized expression in Dictyostelium discoideum enables high-throughput functional read-out. Protein Expr. Purif. 135: 8-15. PubMed
Wiechert, M., Beitz, E. (2017) Mechanism of formate-nitrite transporters by dielectric shift of substrate acidity. EMBO J. 36: 949-958. PubMed
Golldack, A., Henke, B., Bergmann, B., Wiechert, M., Erler, H., Blancke Soares, A., Spielmann, T., Beitz, E. (2017) Substrate-analogous inhibitors exert antimalarial action by targeting the Plasmodium lactate transporter PfFNT at nanomolar scale. PLoS Pathog. 13: e1006172. PubMed
2016
Albers, T., Maniak, M., Beitz, E., von Bülow, J. (2016) The C isoform of Dictyostelium tetraspanins localizes to the contractile vacuole and contributes to resistance against osmotic stress. PLoS One 11: e0162065. PubMed
Holm-Bertelsen, J., Bock, S., Helmstetter, F., Beitz, E. (2016) High-level cell-free production of the malarial lactate transporter PfFNT as a basis for crystallization trials and directional transport studies. Protein Expr. Purif. 126: 109-114. PubMed / Layman summary at Atlas of Science
von Bülow, J., Beitz, E. (2016) Attacking aquaporin water and solute channels of human-pathogenic parasites: new routes for treatment? in Aquaporins in Health and Disease CRC Press
Song, J., Baker, N., Rothert, M., Henke, B., Jeacock, L., Horn, D., Beitz, E. (2016) Pentamidine is not a permeant but a nanomolar inhibitor of the Trypanosoma brucei aquaglyceroporin-2. PLoS Pathog. 12: e1005436. PubMed
2015
Beitz, E., Golldack, A., Rothert, M., von Bülow, J. (2015) Challenges and achievements in the therapeutic modulation of aquaporin functionality. Pharmacol. Ther. 155: 22-35. PubMed
von Bülow, J., Beitz, E. (2015) Number and regulation of protozoan aquaporins reflect environmental complexity. Biol. Bull. 229: 38-46. PubMed
Wu, B., Rambow, J., Bock, S., Holm-Bertelsen, J., Wiechert, M., Blancke Soares, A., Spielmann, T., Beitz, E. (2015) Identity of a Plasmodium lactate/H+ symporter structurally unrelated to human transporters. Nat. Commun. 6: 6284. PubMed
von Bülow, J., Golldack, A., Albers, T., Beitz, E. (2015) The amoeboidal Dictyostelium aquaporin AqpB is gated via Tyr216 and aqpB gene deletion affects random cell motility. Biol. Cell 107: 78-88. PubMed
Geadkaew, A., von Bülow, J., Beitz, E., Tesana, S., Grams, S.V., Grams, R. (2015) Bi-functionality of Opisthorchis viverrini aquaporins. Biochimie 108: 149-159. PubMed
2014
Krenc, D., Song, J., Almasalmeh, A., Wu, B., Beitz, E. (2014) The arginine-facing amino acid residue of the rat aquaporin 1 constriction determines solute selectivity according to its size and lipophilicity. Mol. Membr. Biol. 31: 228-238. PubMed
Rambow, J., Wu, B., Rönfeldt, D., Beitz, E. (2014) Aquaporins with anion/monocarboxylate permeability: mechanisms, relevance for pathogen-host interactions. Front. Pharmacol. 5: 199. PubMed
Song, J., Mak, E., Wu, B., Beitz, E. (2014) Parasite aquaporins: current developments in drug facilitation and resistance. Biochim. Biophys. Acta 1840: 1566-1573. PubMed
Almasalmeh, A., Krenc, D., Wu, B., Beitz, E. (2014) Structural determinants of the hydrogen peroxide permeability of aquaporins. FEBS J. 281: 647-656. PubMed
2013
Zeuthen, T., Alsterfjord, M., Beitz, E., MacAulay, N. (2013) Osmotic water transport in aquaporins. Evidence for a stochastic mechanism. J. Physiol. 591: 5017-5029. PubMed
Müller-Lucks, A., Gena, P., Frascaria, D., Altamura, N., Svelto, M., Beitz, E., Calamita, G. (2013) Preparative scale production and functional reconstitution of a human aquaglyceroporin (AQP3) using a cell free expression system. N. Biotechnol. 30: 545-551. PubMed
Krenc, D., Wu, B., Beitz, E. (2013) Specific aquaporins increase the ammonia tolerance of a Saccharomyces cerevisiae mep1-3 fps1 deletion strain. Mol. Membr. Biol. 30: 43-51. PubMed
Seeliger, D., Zapater, C., Krenc, D., Haddoub, R., Flitsch, S., Beitz, E., Cerda, J., de Groot, B.L. (2013) Discovery of novel human aquaporin-1 blockers. ACS Chem. Biol. 8: 249-256. PubMed
2012
Altmann, K.S., Havemeyer, A., Beitz, E., Clement, B. (2012) Dimethylarginine-dimethylaminohydrolase-2 (DDAH-2) does not metabolize methylarginines. ChemBioChem 13: 2599-2604. PubMed
Müller-Lucks, A., Bock, S., Wu, B., Beitz, E. (2012) Fluorescent in situ folding control for rapid optimization of cell-free membrane protein synthesis. PLoS One 7: e42186. PubMed
Song, J., Almasalmeh, A., Krenc, D., Beitz, E. (2012) Molar concentrations of sorbitol and polyethylene glycol inhibit the Plasmodium aquaglyceroporin but not that of E. coli: involvement of the channel vestibules. Biochim. Biophys. Acta 1818: 1218-1224. PubMed
von Bülow, J., Müller-Lucks, A., Kai, L., Bernhard, F., Beitz, E. (2012) Functional characterization of a novel aquaporin from Dictyostelium discoideum amoebae implies a unique gating mechanism. J. Biol. Chem. 287: 7487-7494. PubMed
2011
Song, J., Wu, B., Beitz, E. (2011) Functional and evolutional implications of natural channel-enzyme fusion proteins. BioMol. Concepts 2: 439- 444. PubMed
Dietz, S., von Bülow, J., Beitz, E., Nehls, U. (2011) The aquaporin gene family of the ectomycorrhizal fungus Laccaria bicolor: lessons for symbiotic functions. New Phytol. 190: 927-940. PubMed
Li, H., Chen, H., Steinbronn, C., Wu, B., Beitz, E., Zeuthen, T., Voth, G.A. (2011) Enhancement of proton conductance by mutations of the selectivity filter of aquaporin-1. J. Mol. Biol. 407: 607-620. PubMed
Wree, D., Wu, B., Zeuthen, T., Beitz, E. (2011) Requirement for asparagine in the aquaporin NPA signature motifs for cation exclusion. FEBS J. 278: 740-748. PubMed
Geadkaew, A., von Bülow, J., Beitz, E., Grams, S.V., Viyanant, V., Grams, R. (2011) Functional analysis of novel aquaporins from Fasciola gigantica. Mol. Biochem. Parasitol. 175: 144-153. PubMed
Mukhopadhyay, R., Mandal, G., Atluri, V.S.R., Figarella, K., Uzcategui, N.L., Zhou, Y., Beitz, E., Ajees, A.A., Bhattacharjee, H. (2011) The role of alanine 163 in solute permeability of Leishmania major aquaglyceroporin LmAQP1. Mol. Biochem. Parasitol. 175: 83-90. PubMed
2010
Wu, B., Song, J., Beitz, E. (2010) Novel channel-enzyme fusion proteins confer arsenate resistance. J. Biol. Chem. 285: 40081-40087. PubMed
Mukhopadhyay, R., Beitz, E. (2010) Metalloid transport by aquaglyceroporins: consequences in the treatment of human diseases. Adv. Exp. Med. Biol. 679: 57-69. PubMed
2009
Wu, B., Steinbronn, C., Alsterfjord, M., Zeuthen, T., Beitz, E. (2009) Concerted action of two cation filters in the aquaporin water channel. EMBO J. 28: 2188-2194. PubMed
Aquaporins (2009) Series: Handbook of Experimental Pharmacology, Vol. 190; Beitz, Eric (Ed.) Springer
Beitz, E., Becker, D., von Bülow, J., Conrad, C., Fricke, N., Geadkaew, A., Krenc, D., Long, H., Song, J., Wree, D., Wu, B. (2009) In vitro analysis and modification of aquaporin pore selectivity. Handb. Exp. Pharmacol. 190: 77-92. PubMed
2008
Kotthaus, J., Schade, D., Muschik, N., Beitz, E., Clement, B. (2008) Structure-activity relationship of novel and known inhibitors of human dimethylarginine dimethylaminohydrolase-1: Alkenyl-amidines as new leads. Bioorg. Med. Chem. 16: 10205-10209. PubMed
Kotthaus, J., Schade, D., Töpker-Lehmann, K., Beitz, E., Clement, B. (2008) N(delta)-methylated L-arginine derivatives and their effects on the nitric oxide generating system. Bioorg. Med. Chem. 16: 2305-2312. PubMed
Wu, B., Altmann, K., Barzel, I., Krehan, S., Beitz, E. (2008) A yeast based phenotypic screen for aquaporin inhibitors. Pflügers Arch. - Eur. J. Physiol. 456: 717-720. PubMed
Bahamontes-Rosa, N., Robin, A., Ambrosio, A.R., Messias-Reason, I., Beitz, E., Flitsch, S.L., Kun, J.F.J. (2008) Monoquaternary ammonium derivatives inhibit growth of protozoan parasites. Parasitol. Int. 57: 132-137. PubMed
2007
Beitz, E. (2007) Jammed traffic impedes parasite growth. Proc. Natl. Acad. Sci. USA 104: 13855-13856. PubMed
Robin, A., Brown, F., Bahamontes-Rosa, N., Wu, B., Beitz, E., Kun, J.F.J., Flitsch, S.L. (2007) Microwave-assisted ring opening of epoxides: a general route to the synthesis of 1-aminopropan-2-ols with anti malaria parasite activities. J. Med. Chem. 50: 4243-4249. PubMed
Wu, B., Beitz, E. (2007) Aquaporins with selectivity for unconventional permeants. Cell. Mol. Life Sci. 64: 2413-2421. PubMed
Bahamontes-Rosa, N., Wu, B., Beitz, E., Kremsner, P.G., Kun, J.F.J. (2007) Limited genetic diversity of the Plasmodium falciparum aquaglyceroporin gene. Mol. Biochem. Parasitol. 156: 255-257. PubMed
2006
Zeuthen, T., Wu, B., Pavlovic-Djuranovic, S., Holm, L.M., Uzcategui, N.L., Duszenko, M., Kun, J.F.J., Schultz, J.E., Beitz, E. (2006) Ammonia permeability of the aquaglyceroporins from Plasmodium falciparum, Toxoplasma gondii and Trypanosoma brucei. Mol. Microbiol. 61: 1598-1608. PubMed
Pavlovic-Djuranovic, S., Kun, J.F.J., Schultz, J.E., Beitz, E. (2006) Dihydroxyacetone and methylglyoxal as permeants of the Plasmodium aquaglyceroporin inhibit parasite proliferation. Biochim. Biophys. Acta 1758: 1012-1017. PubMed
Beitz, E. (2006) Subfamily logos: visualization of sequence deviations at alignment positions with high information content. BMC Bioinformatics 7:313. PubMed
Beitz, E. (2006) Aquaporin water and solute channels from malaria parasites and other pathogenic protozoa. ChemMedChem 1: 587-592. PubMed
Beitz, E., Liu, K., Ikeda, M., Guggino, W.B., Agre, P., Yasui, M. (2006) Determinants of AQP6 trafficking to intracellular sites vs. to the plasma membrane in transfected mammalian cells. Biol. Cell 98: 101-109. PubMed
Beitz, E., Wu, B., Holm, L.M., Schultz, J.E., Zeuthen, T. (2006) Point mutations in the aromatic/arginine region in aquaporin 1 allow passage of urea, glycerol, ammonia and protons. Proc. Natl. Acad. Sci. USA 103: 269-274. PubMed
2005
Beitz, E. (2005) Aquaporins from pathogenic protozoan parasites: structure, function and potential for chemotherapy. Biol. Cell 97: 373-383. PubMed
2004
Uzcategui, N.L., Szallies, A., Pavlovic-Djuranovic, S., Palmada, M., Figarella, K., Boehmer, C., Lang, F., Beitz, E., Duszenko, M. (2004) Cloning, heterologous expression and characterization of three aquaglyceroporins from Trypanosoma brucei. J. Biol. Chem. 279: 42669-42676. PubMed
Beitz, E., Pavlovic-Djuranovic, S., Yasui, M., Agre, P., Schultz, J.E. (2004) Molecular dissection of water and glycerol permeability of the aquaglyceroporin from Plasmodium falciparum by mutational analysis. Proc. Natl. Acad. Sci. USA 101: 1153-1158. PubMed
2003
Pavlovic-Djuranovic, S., Schultz, J.E., Beitz, E. (2003) A single aquaporin gene encodes a water/glycerol/urea facilitator in Toxoplasma gondii with similarity to plant tonoplast intrinsic proteins. FEBS Lett. 555: 500-504. PubMed
Beitz, E., Zenner, H.-P., Schultz, J.E. (2003) Aquaporin mediated fluid regulation in the inner ear. Mol. Cell. Neurobiol. 23: 315-329. PubMed
2002
Ikeda, M. Beitz, E., Kozono, D., Guggino, W.B., Agre, P., Yasui M. (2002) Characterization of aquaporin-6 as a nitrate channel in mammalian cells: Requirement of pore-lining residue threonine-63. J. Biol. Chem. 277: 39873-39879. PubMed
Hansen, M., Kun, J.F.J., Schultz, J.E., Beitz, E. (2002) A single, bi-functional aquaglyceroporin in blood-stage Plasmodium falciparum malaria parasites. J. Biol. Chem. 277: 4874-4882. PubMed
2001
Agre, P., Borgnia, M.J., Yasui, M., Neely, J.D., Carbrey, J., Kozono, D., Beitz, E., Hoffert, J., Leitch, V., King, L.S. (2001) Discovery of the aquaporins and their impact on basic and clinical physiology. Curr. Top. Membr. 51: 1-38.
2000
Beitz, E. (2000) TeXtopo: shaded membrane protein topology plots in LaTeX2e. Bioinformatics 16: 1050-1051. PubMed
Beitz, E., Kumagami, H., Ruppersberg, J.P., Schultz, J. E., Agre, P. (2000) The kidney in the inner ear. in Molecular Biology and Physiology of Water and Solute Transport (S. Hohmann and S. Nielsen edts.): 59-62.
Hansen, M., Beitz, E., Schultz, J. E. (2000) An aquaporin gene in Plasmodium falciparum. in Molecular Biology and Physiology of Water and Solute Transport (S. Hohmann and S. Nielsen edts.): 389-392.
Beitz, E. (2000) TeXshade: shading and labeling of multiple sequence alignments using LaTeX2e. Bioinformatics 16: 135-139. PubMed
1999
Beitz, E., Kumagami, H., Krippeit-Drews, P., Ruppersberg, J. P., Schultz, J. E. (1999) Expression pattern of aquaporin water channels in the inner ear of the rat. Hearing Res. 132: 76-84. PubMed
Kumagami, H., Beitz, E., Wild, K., Zenner, H.-P., Ruppersberg, J. P., Schultz, J. E. (1999) Expression pattern of adenylyl cyclase isoforms in the inner ear of the rat and immunohistochemical localization of calcineurin in the organ of Corti. Hearing Res. 132: 69-75. PubMed
Beitz, E., Schultz, J. E. (1999) Aquaporine - zelluläre Wasserkanäle als therapeutisches Target? Pharm. u. Z. 28: 67-73. PubMed
Beitz, E., Schultz, J. E. (1999) The mammalian aquaporin water channel family: a promising new drug target. Curr. Med. Chem. 6: 465-475. PubMed
Seebacher, T., Beitz, E., Kumagami, H., Wild, K., Ruppersberg, J. P., Schultz, J. E. (1999) Expression of membrane-bound and cytosolic guanylyl cyclases in the rat inner ear. Hearing Res.127: 95-102. PubMed
1998
Kumagami, H., Loewenheim, H., Beitz, E., Wild, K., Schwartz, H., Yamashita, K., Schultz, J. E., Paysan, J., Zenner, H. P., Ruppersberg, J. P. (1998) The effect of anti-diuretic hormone on the endolymphatic sac of the inner ear. Pflügers Arch. - Eur. J. Physiol.436: 970-975. PubMed
Beitz, E., Völkel, H., Guo, Y., Schultz, J. E. (1998) Adenylyl cyclase type 7 is the predominant isoform in the bovine retinal pigment epithelium. Acta Anat. 162: 157-162. PubMed
1996
Völkel, H., Beitz, E., Klumpp, S., Schultz, J. E. (1996) Cloning and expression of a bovine adenylyl cyclase type VII specific to the retinal pigment epithelium. FEBS Lett. 378: 245-249. PubMed